TitleHigh-Throughput Quantitative Genetic Interaction Mapping in the Fission Yeast .
Publication TypeJournal Article
Year of Publication2018
AuthorsRoguev A, Ryan CJ, Hartsuiker E, Krogan NJ
JournalCold Spring Harb Protoc
Volume2018
Issue2
Paginationpdb.top079905
Date Published2018 Feb 01
ISSN1559-6095
Abstract

Epistasis mapping, in which the phenotype that emerges from combining pairs of mutations is measured quantitatively, is a powerful tool for unbiased study of gene function. When performed at a large scale, this approach has been used to assign function to previously uncharacterized genes, define functional modules and pathways, and study their cross talk. These experiments rely heavily on methods for rapid sampling of binary combinations of mutant alleles by systematic generation of a series of double mutants. Epistasis mapping technologies now exist in various model systems. Here we provide an overview of different epistasis mapping technologies, including the pombe epistasis mapper (PEM) system designed for the collection of quantitative genetic interaction data in fission yeast Comprising a series of high-throughput selection steps for generation and characterization of double mutants, the PEM system has provided insight into a wide range of biological processes as well as facilitated evolutionary analysis of genetic interactomes across different species.

DOI10.1101/pdb.top079905
Alternate JournalCold Spring Harb Protoc
PubMed ID28733404
Grant List11809 / / Cancer Research UK / United Kingdom