Title | The Role of Chromatin Density in Cell Population Heterogeneity during Stem Cell Differentiation. |
Publication Type | Journal Article |
Year of Publication | 2017 |
Authors | Golkaram M, Jang J, Hellander S, Kosik KS, Petzold LR |
Journal | Sci Rep |
Volume | 7 |
Issue | 1 |
Pagination | 13307 |
Date Published | 2017 Oct 17 |
ISSN | 2045-2322 |
Abstract | We incorporate three-dimensional (3D) conformation of chromosome (Hi-C) and single-cell RNA sequencing data together with discrete stochastic simulation, to explore the role of chromatin reorganization in determining gene expression heterogeneity during development. While previous research has emphasized the importance of chromatin architecture on activation and suppression of certain regulatory genes and gene networks, our study demonstrates how chromatin remodeling can dictate gene expression distribution by folding into distinct topological domains. We hypothesize that the local DNA density during differentiation accentuate transcriptional bursting due to the crowding effect of chromatin. This phenomenon yields a heterogeneous cell population, thereby increasing the potential of differentiation of the stem cells. |
DOI | 10.1038/s41598-017-13731-3 |
Alternate Journal | Sci Rep |
PubMed ID | 29042584 |
PubMed Central ID | PMC5645312 |
Grant List | R01 EB014877 / EB / NIBIB NIH HHS / United States |