TitleThe Role of Chromatin Density in Cell Population Heterogeneity during Stem Cell Differentiation.
Publication TypeJournal Article
Year of Publication2017
AuthorsGolkaram M, Jang J, Hellander S, Kosik KS, Petzold LR
JournalSci Rep
Volume7
Issue1
Pagination13307
Date Published2017 Oct 17
ISSN2045-2322
Abstract

We incorporate three-dimensional (3D) conformation of chromosome (Hi-C) and single-cell RNA sequencing data together with discrete stochastic simulation, to explore the role of chromatin reorganization in determining gene expression heterogeneity during development. While previous research has emphasized the importance of chromatin architecture on activation and suppression of certain regulatory genes and gene networks, our study demonstrates how chromatin remodeling can dictate gene expression distribution by folding into distinct topological domains. We hypothesize that the local DNA density during differentiation accentuate transcriptional bursting due to the crowding effect of chromatin. This phenomenon yields a heterogeneous cell population, thereby increasing the potential of differentiation of the stem cells.

DOI10.1038/s41598-017-13731-3
Alternate JournalSci Rep
PubMed ID29042584
PubMed Central IDPMC5645312
Grant ListR01 EB014877 / EB / NIBIB NIH HHS / United States